مقاله تفکیک DNA ارگانیسم اصلاح شده ژنتیکی و تصفیه با نانو ذرات مغناطیسی

Summary DNA extraction is always an important and key step in bioanalytical fields for target nucleic acid detection.

Traditional organic solvent-based extraction methods are toxic and time-consuming. In this work, we report

a systematic study of magnetic nanoparticles (MNPs) as probes for genomic DNA extraction from

genetically modified organism (GMO) plants. Different surface-functionalised MNPs with controllable

diameters have been used to extract the genomic DNA directly from GMO plants for detections. Systematic

comparison of the results obtained under different extraction conditions has indicated that carboxylmodified

MNPs with smaller diameters are more suitable for genomic DNA extractions. The successful

qualitative detection of GMO and non-GMO products based on the MNP extracted DNA is also achieved

and discussed in this article. In view of the advantages of magnetic extraction, such as nontoxicity, ease of

operation, and rapid and high throughput, this systematic research has demonstrated the great potential of

the method and provides theoretical guidance for practical MNP applications.

Keywords DNA detection, DNA extraction, functional groups, genetically modified organism, magnetic nanoparticles

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